Bacterial Culture Collection Methods
by
mike_rogers
—
last modified
March 16, 2009 01:46 PM
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UNC Hospitals McLendon Clinical Laboratories 101 Manning Drive Chapel Hill, NC 27514 |
| Specimen Type | Acceptable Specimen Types | Additional Information | Transport | Comments |
| Aerobic Culture | Surface Swabs, Drainage, Intra-vascular Catheter Tips, Gastric Aspirates, Irrigation/Wash Fluids, Female/Male Genital Tract, Placental Swabs, Throat Culture, etc. | Swabs are to be delivered in Transport Swabs (Lawson# 050441 - standard or Lawson# 050440 - mini-tip). For the Pediatric Screening Clinic only, throat cultures should be collected on a Transport Double Swab (Lawson# 050439) Fluid specimens may be delivered in Luer-lock capped syringe WITHOUT NEEDLE or in other suitable specimen container. Luer caps avaialble from CD (Lawson# 000519) |   
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| Anaerobic Culture | Internal body fluids, Aspirates, Tissue Biopsies, Surgical Swabs of Internal Organs and Other Internal Sites. | Fluid Specimens are to be delivered in a Luer-lock capped syringe WITHOUT NEEDLE Surgical Swabs are to be delivered in Transport Swabs (Lawson# 050442) | 
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| Ascitic Fluid | BacT/Alert FA if <4mL (1) BacT/Alert FA bottle, >6mL divide evenly into 2 FA bottles ABSOLUTE VOLUMES: | |||
| Bartlett Bronchial Brush | Special Transport Medium available in Bronchoscopy Suite | |||
| Blood Culture | Order via floor-order entry system. Collection by Phlebotomy Services (6-2446) | |||
| Bronchial Alveolar Lavage | Deliver in Luer-lock capped syringe WITHOUT NEEDLE or in other suitable sterile container | d | ||
| Fluids | 1-2 ml in a sterile container | |||
| NP Aspirate | Place the #5 french feeding catheter containing the specimen into a sterile container. Bring to the lab immediately | |||
| Sputum | See Sputum Culture Collection Methods | |||
| Tissues | Place in sterile container without medium. Bring to lab immediately. Do not allow to dry out. |
