Tube Guide

by mike_rogers — last modified September 03, 2009 12:12 PM

UNC Hospitals
McLendon Clinical Laboratories
101 Manning Drive
Chapel Hill, NC 27514

PHLEBOTOMY SERVICES

EVACUATED BLOOD COLLECTION TUBE GUIDE.
Listed in Order of Draw for Vacutainer and Syringe Collections.
STOPPER COLOR	CONTENTS	VOL.	USES/COMMENTS
Blood Culture Bottles are ALWAYS drawn prior to other labs to reduce contamination. .
Royal Blue	No additive (serum); special glass and stopper material	7.0 mL	Most drug levels, toxicology screens, and trace elements
Red	No additive	7.0 mL	Cryoglobulins and CH₅₀
Light Blue	3.2% Sodium Citrate	4.5 mL	PT, PTT, TCT, CMV buffy coat, Factor Activity Tube MUST be filled 100% - No exceptions!
Gold Top (Serum Separator, "SST")	Contains separating gel and clot activator	6.0 mL	Most chemistry, endocrine and serology tests, including Hepatitis and HIV
Light Blue - Yellow Label on Tube	Thrombin	2.0 mL	For FDP test ONLY; Obtain tube from Core Lab Coag; Allow to Clot
Green	Sodium heparin(100 USP Units)	5.0 mL	Ammonia, Lactate, HLA Typing
Tan	K² EDTA	5.0 mL	Lead levels
Yellow	ACD Solution A consists of trisodium citrate, citric acid and dextrose	8.5 mL	DNA Studies, HIV Cultures
Pink	(K₂)EDTA	Draws 6 mL - Minimum 4 mL	Blood type & Screen, Compatibility Study, Direct Coombs HIV Viral Load
Pearl Top (Plasma Preparation, "PPT")	Separating gel and (K₂)EDTA	4.0 mL	Adenovirus PCR Toxoplasma PCR HHV-6 PCR
Lavender ("Purple")	(K₂)EDTA	3.0 mL	CBC/Diff/Retic/Sed Rate, FK506, Cyclosporin, Platelet Ab, Coombs, Flow Cytometry

NOTE: All tubes sterile.

Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender. If a coag tube (light blue) is the only tube or the first tube to be drawn, a 5 mL discard tube must be drawn first.

(see also Blood Culture Collection Guide)
SEAL COLOR	BOTTLE TYPE	VOL	USE/COMMENTS
Green	BacT/Alert^® Aerobic FA	Optimal: 10mL	Aerobic "FAN" broth, with activated charcoal to remove potential growth inhibitors. Used in combination with Purple Top (Anaerobic) bottle for adult routine blood culture, and with Wampole Isolator^™ Tube for Rare Pathogen cultures.
Purple	BacT/Alert^® Anaerobic SN	Optimal: 10mL	Standard anerobic broth. Used in combination with Blue Top (Aerobic) bottle for adult routine blood culture.
Yellow	BacT/Alert^® Pediatric PF	Maximum: 4mL	Pediatric volume aerobic "FAN" broth, with activated charcoal to remove potential growth inhibitors.
Black	MB/BacT^® Blood	Maximum: 5 mL	For Mycobacterial blood cultures
Black/Yellow	Wampole Isolator^™ Tube	10mL	Contains lytic and cushioning agents. Sample is processed and inoculated directly to selective &/or enriched media. Used in combination with Green Top (aerobic) bottle for unusual "rare" pathogens, particularly intracellular organisms. Specify suspected organism PRIOR to ordering.

Document Actions

Print this

Sections

Tube Guide

PHLEBOTOMY SERVICES

Document Actions