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Tube Guide

Last modified by  ivette_marrero  on  February 17, 2015 12:27 PM


UNC Hospitals
McLendon Clinical Laboratories
101 Manning Drive
Chapel Hill, NC 27514
 

McLendon Clinical Laboratories

 

PHLEBOTOMY SERVICES

EVACUATED BLOOD COLLECTION TUBE GUIDE.
Listed in Order of Draw for Vacutainer and Syringe Collections.
STOPPER COLOR SYMBOL CONTENTS VOL. USES/COMMENTS

Blood Culture Bottles are ALWAYS drawn prior to other labs to reduce contamination. .

Royal Blue Royal Blue Top No additive (serum); special glass and stopper material 7.0 mL Most drug levels, toxicology screens, and trace elements
Red Red Top No additive 7.0 mL Cryoglobulins and CH50
Light Blue Light Blue Top 3.2% Sodium Citrate 4.5 mL PT, PTT, TCT, CMV buffy coat, Factor Activity
Tube MUST be filled 100% - No exceptions!
Gold Top (Serum Separator, "SST") Gold Top (SST) Contains separating gel and clot activator 6.0 mL Most chemistry, endocrine and serology tests, including Hepatitis and HIV
Light Blue -
Yellow Label on Tube
FDP (Light Blue Top with Yellow Label) Thrombin 2.0 mL For FDP test ONLY; Obtain tube from Core Lab Coag; Allow to Clot
Green Green Top Sodium heparin(100 USP Units) 5.0 mL Ammonia, Lactate, HLA Typing
Tan Tan Top

K2 EDTA

 

5.0 mL Lead levels
Yellow Yellow Top ACD Solution A consists of trisodium citrate, citric acid and dextrose 8.5 mL DNA Studies, HIV Cultures
Pink Pink Top (K2)EDTA Draws 6 mL - Minimum 4 mL Blood type & Screen, Compatibility Study, Direct Coombs
HIV Viral Load
Pearl Top (Plasma Preparation, "PPT") Pearl Top Separating gel and (K2)EDTA 4.0 mL Adenovirus PCR
Toxoplasma PCR
HHV-6 PCR
Lavender ("Purple")  Lavender (Purple) Top (K2)EDTA 3.0 mL CBC/Diff/Retic/Sed Rate, FK506, Cyclosporin, Platelet Ab, Coombs, Flow Cytometry

 NOTE:  All tubes sterile.

Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender. If a coag tube (light blue) is the only tube or the first tube to be drawn, a 5 mL discard tube must be drawn first.

 
Blood Culture Options (Specify Culture Type when Ordering)

 

Culture Type Patient Population Test ID Test # BottleType/Comments  
Culture, Blood, Routine

All Patients not Meeting Criteria Below

 

 

 

 

NOTE:  This culture will recover
HACEK organisms with no
change in collection
or incubation  conditions.
If Brucella or Francisella are
in the differential, extended
incubation may be required. 
Please contact the
Microbiology Lab (966-4056).

 

BROU 9500

 
Bactec Plus Aerobic F

BD BACTEC Plus Aerobic/F 
(Grey Cap/Blue Ring)
(Optimal Volume 8-10 mL)
Lawson# 052346(UOM box of 50 bottles) 
 

Bactec Lytic Anaerobic F

BD BACTEC Anaerobic Lytic/10
(Purple Cap/Maroon Ring)
(Optimal Volume 8-10  mL)
Lawson# 052347
(UOM box of 50 bottles)
 
Culture, Blood, AFB HIV+  patients with CD4 Count <100; 
Suspected MAC infections
AFBB 9684

 

 Bactec Myco FBactec Myco F

BD BACTEC Myco/F Lytic
(White Cap/Red Ring)
Available from Phlebotomy Lab
(Optimal Volume 1-5 mL)
 
 
Culture, Blood, Pediatric Patients Weighing <40 kg BPED 9548

 

 

Bactec Plus Aerobic F

 

 
BD BACTEC Plus aerobic/F
(Grey Cap/Blue Ring)
(Optimal Bolume 8-10 mL)
For Patients <20 Kg, 1-4 mL is acceptable
For Patients 20-40 Kg, 4-8 mL in one bottle.
For Patients >40 Kg (See Routine above)
 
 
Blood Culture Mould
Suspected infections due to
dimorphic  (endemic) fungi,
Fusarium sp., Trichosporon sp.,
Malassezia furfur (Specify at Collection).
BPATH 9507

 

Isolator Tube

Isolator Yellow Top
(10 mL Volume)
Available from Plebotomy
 
 
Bactec Plus Aerobic F
 
BD BACTEC Plus Aerobic/F 
(Grey Cap/Blue Ring)
(Optimal Volume 8-10 mL)
Lawson# 052346
(UOM box of 50 bottles)
 
Bactec Myco F
 
BD BACTEC Myco/F Lytic
(White Cap/Red Ring)
Available from Phlebotomy Lab
(Optimal Volume 1-5 mL)
 
 

 

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